Referring to a group as “guys” usually means a group of men or a mixed-gender group, since describing a group of women as guys, as in “The Pussycat Dolls are a bunch of guys”, suggests that they are male, and is generally viewed as incorrect or inaccurate in that usage. In contrast, the all-male band Green Day could. "Guys" can be used in English as gender neutral to refer to a group of mixed gender. You will even hear women refer to other women as "guys." The closest linguistic equivalent with a feminine tilt would be "gals". "Guys and gals" is a rather informal variant of "ladies and gentlemen". (Note the reverse order.). "You guys" is a familiar, all-inclusive way of addressing a group of men or women directly. That said, there are some important distinctions you must understand: " You guys" is more likely to be said in women => women or men => men or women => men or mixed-group => mixed-group contexts. It is less.